| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Carlinville | A | Ashby | Kimbro | Pope | Wiese | 57.04 |
| 2 | Litchfield | A | Ray | Stewart | Guinn | Braasch | 53.50 |
| 3 | New Berlin | A | Cowhick | Brewer | Workman | Periman | 51.87 |
| 4 | Pana | A | Woolard | Rhoades | Cutler | Chronister | 52.08 |
| 5 | Pleasant Plains | A | Shaffer | Wilson | McCann | Bergner | 54.60 |
| 6 | Central A & M | A | Caudle | Younker | Dial | Woods | 1:00.04 |