| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Okawville | A | Frantz | Auer | Shubert | Shubert | NT | |
| 2 | Chester | A | Bicket | Cowan | Rodriguez Duran | Steele | 57.04 | |
| 3 | Dupo | A | Morning | Schroeder | Jordan | Jordan | 48.96 | |
| 4 | Wood River (East Alton-W.R.) | A | Skinner | Lane | Marshall | Burch | 46.50 | |
| 5 | Red Bud | A | Baldwin | Hall | Krack | Schwartzkopf | 47.58 | |
| 6 | New Athens | A | Mathis | Wilson | Dachsteiner | Mazariego | 49.94 | |
| 7 | Lebanon | A | Ells | Wilson | Wright | Harris | 1:45.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sparta | A | Williams | Greer | Williams | Lofton | 45.64 | |
| 2 | Salem | A | Greene | Tennyson | Ettress | Thornton | 45.02 | |
| 3 | Freeburg | A | Banks | Jarvis | Murphy | Thompson | 44.86 | |
| 4 | Roxana | A | Coles | Wieneke | Osmoe | McBride | 43.79 | |
| 5 | Steeleville | A | Reitz | Buch | Cratch | Wasson | 44.72 | |
| 6 | Columbia | A | Kronk | Nelson | Donovan | Scott | 44.90 | |
| 7 | Trenton (Wesclin) | A | Hendrix | Evans | Gruenke | Kapp | 45.20 | |
| 8 | Breese (Central) | A | Shubert | Revermann | Attaway | Rogers | 45.76 |