| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Chester | A | Cowan | Rodriguez Duran | Hopkins | Caron | NT | |
| 3 | Steeleville | A | Kranz | Davis | Moore | Bockhorn | 1:46.33 | |
| 4 | Breese (Central) | A | Vetter | Attaway | Schiefer | Jansen | 1:42.37 | |
| 5 | Trenton (Wesclin) | A | Monical | Waggoner | Bateman | Evans | 1:44.00 | |
| 6 | Okawville | A | Hasheider | Auer | Jackson | Shubert | DQ |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Columbia | A | Fromme | Donovan | Foxx | Buelteman | 1:40.20 | |
| 2 | Red Bud | A | Wagner | Hill | Schlueter | Baldwin | DQ | |
| 3 | Freeburg | A | Jarvis | Merz | Murphy | Thompson | 1:35.16 | |
| 4 | Roxana | A | Wells | Wieneke | Osmoe | McBride | 1:34.47 | |
| 5 | Salem | A | Ettress | Mayes | Greene | Thornton | 1:35.00 | |
| 6 | Sparta | A | Williams | Williams | Woods | Lofton | 1:36.49 | |
| 7 | Wood River (East Alton-W.R.) | A | Skinner | Marshall | Gould | Burch | 1:39.37 | |
| 8 | Dupo | A | Ross | Schroeder | Jordan | Jordan | 1:41.59 |