| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rochester | A | Browning | Davis-Hill | Johnson | Tom | 55.68 | |
| 2 | Jerseyville (Jersey) | A | Yates | Leslie | Yamnitz | Trexler | 54.84 | |
| 3 | Roxana | A | Daugherty | Lohman | Eads | Carlisle | 53.91 | |
| 4 | Taylorville | A | Ward | Davis | Repscher | Althoff | 53.00 | |
| 5 | Highland | A | Pollard | Henschen | Thomas | Maas | 53.14 | |
| 6 | Wood River (East Alton-W.R.) | A | Minner | Brown | Nguyen | Truax | 54.10 | |
| 7 | Greenville | A | Bellegante | Rohr | Neely | DeBlois | 55.54 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Breese (Central) | A | Morris | Taylor | Benhoff | Bassler | 52.26 | |
| 2 | Troy (Triad) | A | DiRosa | Cissell | Brown | Witham | 51.35 | |
| 3 | Springfield (Sacred Heart-Gr | A | Bartletti | Paul | Scarlette | Redpath | 49.39 | |
| 4 | Cahokia (H.S.) | A | Austin | Johnson | Tucker | Hayes | 48.70 | |
| 5 | East St. Louis (Sr.) | A | McClain | Jones | Graham | Glenn | 48.73 | |
| 6 | Springfield (Southeast) | A | Lowery | Jackson | Howze | Hobson | 51.14 | |
| 7 | Springfield (Lanphier) | A | Lewis | Walker | Williams | Patterson | 52.04 | |
| 8 | Belleville (Althoff Catholic | A | Thorpe | Cunningham | Distler | Distler | 52.97 |