| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Jerseyville (Jersey) | A | Allen | Brooks | Finkes | Soffray | 4:45.69 | |
| 3 | Troy (Triad) | A | Gough | Niehaus | Brown | Kitzmiller | 4:30.54 | |
| 4 | Springfield (Southeast) | A | Shelby | Singleton | Howze | Lowery | 4:26.84 | |
| 5 | Breese (Central) | A | Detmer | Tebbe | Rakers | Morris | 4:30.14 | |
| 6 | Belleville (Althoff Catholic | A | Reyes-Cruz | Distler | Nelson | Distler | 4:37.38 | |
| 7 | Wood River (East Alton-W.R.) | A | Nguyen | Minner | Sanders | Truax | 5:16.86 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Springfield (Lanphier) | A | Watson | Rynsburger | Walker | Patterson | 4:18.63 | |
| 2 | Cahokia (H.S.) | A | Tucker | Millsap | Moncelli | Brooks | 4:16.69 | |
| 3 | Highland | A | Maas | Thomas | Frey | Pace | 4:12.01 | |
| 4 | Springfield (Sacred Heart-Gr | A | Bartletti | Rolf | Fleischli | Scarlette | 4:05.25 | |
| 5 | East St. Louis (Sr.) | A | Hawkins | Graham | Lampley | Glenn | 4:09.18 | |
| 6 | Rochester | A | Zeibert | Hicks | Zeibert | Zeibert | 4:13.14 | |
| 7 | Taylorville | A | Lowis | Goodman | Goodman | Odam | 4:17.50 | |
| 8 | Bethalto (Civic Memorial) | A | Hannaford | Vaughn | Mann | Meiser | 4:22.70 |