| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Zada DEATHERAGE | FR | Jacksonville (H.S.) | 1:00.64 | |
| 2 | Claire FLATT | FR | Rochester | 58.21 | |
| 3 | Autumn BOUNDS | FR | Springfield (Southeast) | 57.69 | |
| 4 | Cristina REYES-CRUZ | FR | Belleville (Althoff Catholic | 56.68 | |
| 5 | Kaylee SHIELDS | FR | Bethalto (Civic Memorial) | 57.00 | |
| 6 | Cheyenne CHOAT | SR | Highland | 57.78 | |
| 7 | Kennadi HEBEL | SR | Belleville (Althoff Catholic | 1:00.18 | |
| 8 | Mary NGUYEN | SR | Wood River (East Alton-W.R.) | 1:00.75 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Rebecca RYAN | JR | Rochester | 56.50 | |
| 2 | Falynn MEYER | SO | Breese (Central) | 55.17 | |
| 3 | Lillian HENRICHS | FR | Breese (Central) | 54.03 | |
| 4 | Jazmine MONCELLI | FR | Cahokia (H.S.) | 53.12 | |
| 5 | Inara SMITH | FR | Troy (Triad) | 53.86 | |
| 6 | Alexis GURLEY | FR | Highland | 54.39 | |
| 7 | Amirah LAWSON | SO | Springfield (Southeast) | 55.90 | |
| 8 | Arlynn WEBER | FR | Jerseyville (Jersey) | 56.57 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Skylar WOODS | FR | East St. Louis (Sr.) | 52.01 | |
| 2 | Sophia PAUL | SO | Springfield (Sacred Heart-Gr | 50.32 | |
| 3 | Andrianna MILLSAP | SR | Cahokia (H.S.) | 49.79 | |
| 4 | Gina TRUAX | FR | Wood River (East Alton-W.R.) | 48.03 | |
| 5 | Macie ROLF | SO | Springfield (Sacred Heart-Gr | 48.98 | |
| 6 | Mikayla NIEHAUS | SO | Troy (Triad) | 50.01 | |
| 7 | Jaylah HARRIS | FR | East St. Louis (Sr.) | 50.42 | |
| 8 | Emlyn MATEER | FR | Taylorville | 53.03 |