| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Civic Memorial | A | Silva | Wooten | Atteberry | Fonrodona | 46.80 | |
| 2 | Maryville Christian | A | Ury | King | Malone | King | 46.28 | |
| 3 | Lanphier | A | Robinson | Harris | Holmes | Bass | 44.37 | |
| 4 | North Point | A | Trennepohl | Pyatt | Wicklund | Trennepohl | 44.03 | |
| 5 | Rochester | A | Mrozowski | Hobson | Huet | Pletsch | 44.30 | |
| 6 | University (Normal) | A | Zhang | Nicolas | Lawal | Bair | 46.20 | |
| 7 | Mt. Vernon | A | Bates | Hawkins | Zirkelbach | Bell | DNS | |
| 8 | Collinsville | A | Nabors | Munoz | Patterson | Harper | 46.92 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Mac | A | Bricker | Hogan | Bolleto | Brown | 43.95 | |
| 2 | Glenwood | A | Rayl | Marcordes | Sgro | Saunders | 43.60 | |
| 3 | O'Fallon | A | Johnson | Dowell | Green | Mims | 43.22 | |
| 4 | Triad | A | Darby | Crockarell | Warren | Yohannes | 42.34 | |
| 5 | Edwardsville | A | Brnfre | Lakatos | Wilson | Lane | 42.75 | |
| 6 | Belleville West | A | Martin | Jackson | Fountian | Abram | 43.30 | |
| 7 | Belleville East | A | Smith | Howard | Martin | Liddell | DNS | |
| 8 | Roxana | A | Coles | Wieneke | Osmoe | McBride | 44.02 |