| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mt. Vernon | A | Bates | Hawkins | Zirkelbach | Bell | 1:39.50 | |
| 2 | Rochester | A | Pletsch | Barton | Olson | Hobson | 1:35.70 | |
| 3 | Lanphier | A | Harris | Holmes | Hall-Burton | Horne | 1:35.42 | |
| 4 | University (Normal) | A | Zhang | Nicolas | Lawal | Bair | 1:35.00 | |
| 5 | Roxana | A | Wells | Wieneke | Osmoe | McBride | 1:35.20 | |
| 6 | Collinsville | A | Munoz | Yarbough | Strong | Patterson | 1:35.50 | |
| 7 | Civic Memorial | A | Silva | Turner | Atteberry | Fonrodona | 1:39.50 | |
| 8 | Maryville Christian | A | Malone | Coulby | Paul | Abert | 2:00.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Mac | A | Bricker | Bolleto | Hogan | Brown | DQ | |
| 2 | Glenwood | A | Marcordes | Downen | Sgro | Saunders | 1:31.68 | |
| 3 | Cahokia | A | Midgett | Rayford | Moore | Deloach | 1:29.68 | |
| 4 | Belleville West | A | Sutton | Jackson | Henderson | Smith-Johnson | 1:29.31 | |
| 5 | Edwardsville | A | Wilson | Brnfre | Lane | Robinson | 1:29.62 | |
| 6 | Triad | A | Darby | Crockarell | Warren | Yohannes | DNS | |
| 7 | Belleville East | A | Smith | Fulton-Wilson | Martin | Howard | 1:32.50 | |
| 8 | O'Fallon | A | Dowell | Johnson | Coley | Mims | 1:34.91 | St. Clair County Meet |