| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roxana | A | Schmidt | Doyle | Ponce | McBride | 3:45.00 | |
| 2 | North Mac | A | Hogan | Fatheree | Fatheree | Downs | 3:38.20 | |
| 3 | Glenwood | A | Saunders | Tode | Morsch | Verenski | 3:36.00 | |
| 4 | East Alton-Wood River | A | Skinner | Lane | Marshall | Burch | DNS | |
| 5 | Lanphier | A | Bass | Smith | Horne | Hall-Burton | 3:35.52 | |
| 6 | Rochester | A | Huet | Mrozowski | Gibson | Trello | DNS | |
| 7 | Triad | A | Yohannes | Warren | Pace | Venhaus | 3:39.06 | |
| 8 | Maryville Christian | A | Ury | Abert | Jacob | Gillihan | 3:49.30 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Munoz | Patterson | Thorps-Watt | Peterson | 3:33.00 | |
| 2 | Mascoutah | A | Feldt | Williams | Ellsworth | Killian | 3:31.20 | St. Clair County Meet |
| 3 | Belleville East | A | Smith | Howard | Fulton-Wilson | Gardner | 3:30.05 | |
| 4 | Edwardsville | A | Valdez | Wilson | Lane | Heavens | 3:27.45 | |
| 5 | Belleville West | A | Fountian | Mcclean | Henderson | Abram | 3:30.00 | |
| 6 | Alton | A | Singleton | Kotzamanis | Harris | McClaine | 3:30.90 | |
| 7 | University (Normal) | A | Alcorn | Nicolas | Hart | Immanuel | 3:32.48 | |
| 8 | North Point | A | Trennepohl | Lacke | Trennepohl | Wicklund | 3:35.00 |