| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Alton | B | Palmer | Hogan | West | Omlor | 49.00 | |
| 3 | Triad | B | Davis | Lamb-Carraway | Dudley | Coleman | 47.40 | |
| 4 | O'Fallon | A | Pasley | Hunter | Ross | Fisher | 46.50 | |
| 5 | Madison | A | Hinds | Willimas | Hughes | Brown | 46.90 | |
| 6 | Collinsville | A | Hale | Strong | Holmes | Hendrickson | 48.00 | |
| 7 | Collinsville | B | Behne | Farris | Kamau | Leflore | 51.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cahokia | A | Midgett | Taylor | Pennington | Morris | 45.70 | |
| 2 | Alton | A | Rounds | Johnson | Holeman | White | 45.40 | |
| 3 | Edwardsville | A | Word | Stemmons | Lyles | Smith | 44.13 | |
| 4 | Belleville East | A | Smith | Dewalt | Hardy | Mitchell | 43.50 | |
| 5 | Belleville West | A | Onyemelukwe | Martin | Cole | Sutton | 44.00 | |
| 6 | Belleville East | B | Moise | Young | Jones | Fuller | 44.20 | |
| 7 | Granite City | A | Brokaw | Dean | Webb | Rodgers-Parott | 45.56 | |
| 8 | Triad | A | Hard | Lightner | Vanzandt | Dempsey | 45.90 |