| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Belleville West | A | Hill, Ni'el | Cason, Ni'Zarien | Carter, Lamondre | Buchanan, Deshon | 1:29.74 |
| 2 | Alton | A | Dobbins, Tony | Henderson, Harrison | Bumpers, Jonathan | Stegall, Keenan | 1:29.74 |
| 3 | Edwardsville | A | Jacobs, Seth | Queen, Jason | Booker, Donovan | Torres, Daval | 1:29.93 |
| 4 | Cahokia | A | CROSBY, CHRISHWAN | RAYMOND, CHRIS | ROBERSON, BRANDON | WEST, DARREON | 1:30.00 |
| 5 | Harrisburg | A | Crim-Harbison, Corbin | Marler, Tyler | Lane, Dustin | Neal, Niko | 1:31.00 |
| 6 | Collinsville | A | Crone, Bailey | Cooper-Hodges, Amron | Romanik, Rhett | Hammel-Brown, Zach | 1:31.84 |
| 7 | Marion | A | Whitehorn, Trevell | Cambric, Xavier | Bryant, Levi | Adkinson, Stuart | 1:34.40 |
| 8 | Waterloo | A | Thier, Travis | Bean, Antonio | Simunich, Garrett | Howard, Ian | 1:34.90 |
| 9 | Granite City | A | Bell, Wilyonde | Allen, Ron | Williba, Kendrick | Hall, Rashaun | 1:35.00 |
| 10 | Highland | A | Geiger, Mason | Thole, Grant | Wolf, Seby | DeProw, Chase | 1:36.90 |
| 11 | Jacksonville | A | Bonner, Andrew | Luetz, Ben | Bunfill, Dylan | Fernandes, Dylan | 1:37.00 |
| 12 | Jersey | A | Hall, Kevin | Bertman, Alex | Hansen, Kirklyn | Marshall, Dylan | 1:37.00 |
| 13 | Mascoutah | A | Kimble, Hason | Powell, Jawuan | Moseley, Tre | Garlington, Gerik | 1:37.64 |
| 14 | Triad | A | Curtis, Malik | Mitchell, Daquavius | Mitchell, Noah | Brunton, Collin | 1:38.20 |
| 15 | Springfield Southeast | A | Meek, Evan | Ashford, Deshaun | Hill, Vincent | Readus, Dontairius | 1:42.18 |