| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Cahokia | A | 3:18.00 | ||||
| 2 | Marion | A | 3:25.97 | ||||
| 3 | Alton | A | Stegall, Keenan | Lewis, Kyle | Dobbins, Tony | Cummings, Kelvin | 3:28.46 |
| 4 | Waterloo | A | Moehrs, Greysan | Hays, Noah | Rusteberg, Blake | Kreher, Jon | 3:29.13 |
| 5 | Edwardsville | A | Giacobbe, Tommy | Romano, Franky | Harlan, Darryl | Booker, Donovan | 3:31.00 |
| 6 | Granite City | A | O'Keefe, Will | Tindall, Tyler | Allen, Ron | Smith, Jordan | 3:31.00 |
| 7 | Mascoutah | A | Ferguson-Scott, Christopher | Johnson, Brandon | Ignacio, Isaiah | Crossen, Christopher | 3:32.09 |
| 8 | Highland | A | 3:32.80 | ||||
| 9 | Triad | A | 3:34.20 | ||||
| 10 | Belleville West | A | Carter, Lamondre | Evans, Tommie | Cason, Ni'Zarien | Cox, Jaylen | 3:34.90 |
| 11 | Jersey | A | Ross, Lucas | Hall, Kevin | Loepker, Nick | Shaw, A.J. | 3:37.00 |
| 12 | Fort Zumwalt West | A | Hoffmann, Tyler | Vaccaro, Marshall | Ritchie, Brendan | Baze, Tristan | 3:37.40 |
| 13 | Harrisburg | A | Bowling, Chandler | Williams, Chris | Neal, Niko | Smith, Hunter | 3:42.80 |
| 14 | Jacksonville | A | Bonner, Andrew | Luetz, Ben | Fatheree, Ryan | sexton, clayton | 3:45.00 |
| 15 | Collinsville | A | Autenreith, Will | Garth, Juan | Brown, Daniel | Carter, Seth | 3:46.00 |
| 16 | Springfield Southeast | A | Smith, Makavelli | Hacker, Jason | Jones, Opeoluwa | Logan, Michael | 3:48.41 |
| 17 | Springfield Southeast | A | 4:05.32 |