| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Cahokia | A | LYONS, TYRAN | MCGEE, CHRIS | GUFFEY, GABRIEL | STANLEY, CHRIS | 8:03.00 |
| 2 | Belleville West | A | Cason, Hunter | Ernst, Nick | Gichuru, Brian | Reed, Jimmy | 8:08.00 |
| 3 | Waterloo | A | Hays, Noah | Schwartz, Jake | Rusteberg, Blake | Matthews, Jesse | 8:20.00 |
| 4 | Alton | A | Macias, Arie | Cummings, Kelvin | Davis, Alexander | Northway, Nick | 8:29.94 |
| 5 | Granite City | A | O'Keefe, Will | Allen, Ron | Riggins, Juwan | O'Keefe, Andrew | 8:30.00 |
| 6 | Mascoutah | A | Johnson, Brandon | Little, Kyle | Moseley, Tre | Zurbrugg, Parker | 8:30.30 |
| 7 | Marion | A | Young, Zach | Melvin, Ryan | McPhail, Julian | Surburg, Timothy | 8:32.00 |
| 8 | Highland | A | Portell, Brode | Smart, Hayden | Sexton, Bryce | Schrage, Brock | 8:35.93 |
| 9 | Jersey | A | Reynolds, Nick | Loepker, Nick | Wendell, Mark | Roach, Mike | 8:40.00 |
| 10 | Edwardsville | A | Schoenthal, Jacob | Hennig, Wyatt | Potter, Holden | McCormick, Sam | 9:05.00 |
| 11 | Springfield Southeast | A | Meek, Austin | Ayers, Braedyn | Jones, Opeoluwa | White, Josh | 9:25.74 |
| 12 | Jacksonville | A | Covell, Jacob | Fatheree, Ryan | Roegge, Zach | Bonner, Andrew | 9:30.00 |
| 13 | Triad | A | Knopf, Zach | D'Antonio, Ryan | Plantz, Andrew | Barker, Isaiah | 9:45.00 |