| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Jacksonville | A | James, Noah | Luetz, Ben | Bunfill, Dylan | Fernandes, Dylan | 46.32 |
| 2 | Springfield Southeast | A | Meek, Evan | Nix, Da'reco | Readus, Dontairius | Sampson, Rashad | 45.73 |
| 3 | Jersey | A | Hansen, Kirklyn | Goetten, Mitch | Bertman, Alex | Marshall, Dylan | DNF |
| 4 | Waterloo | A | Kreher, Jon | Bean, Antonio | Simunich, Garrett | Thier, Travis | 44.82 |
| 5 | Mascoutah | A | Ignacio, Isaiah | Ferguson-Scott, Christopher | Kimble, Hason | Garlington, Gerik | 45.10 |
| 6 | Granite City | A | Bell, Wilyonde | Bobo, Raishon | Williba, Kendrick | Hall, Rashaun | 45.50 |
| 7 | Highland | A | Geiger, Mason | Wolf, Seby | DeProw, Chase | Teipe, Sam | 45.74 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Crone, Bailey | Carter, Seth | Cooper-Hodges, Amron | Hammel-Brown, Zach | 43.74 |
| 2 | Alton | A | Henderson, Harrison | Dobbins, Tony | Bumpers, Jonathan | Stegall, Keenan | 43.24 |
| 3 | Edwardsville | A | Swanson, Matt | Giacobbe, Tommy | Tincher, Devonte' | Jacobs, Seth | 42.86 |
| 4 | Marion | A | Sullivan, Darryl | Rakers, Luke | Cambric, Xavier | Porter, Aaron | 42.80 |
| 5 | Belleville West | A | Hill, Ni'el | Buchanan, Deshon | Carter, Lamondre | Lewis, Anthony | 42.84 |
| 6 | Cahokia | A | CROSBY, CHRISHWAN | RAYMOND, CHRIS | ROBERSON, BRANDON | WELLS, KEONDRE | 43.00 |
| 7 | Harrisburg | A | Marler, Tyler | Lane, Dustin | Rightnowar, Cody | Crim-Harbison, Corbin | 43.30 |
| 8 | Triad | A | Qualls, Cody | Nickerson, Clayton | Lawson, Sawyer | Sparks, Eddie | 43.90 |