| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Valparaiso | A | Justison, Taylor | Donahue, Maranda | Recker, Oliva | Nash, Peyton | NT |
| 4 | Detroit Mercy | B | Measel, Rebecca | Harden, Rebekah | Sanderson, Allison | Carnathan, Alexandra | 4:12.00 |
| 5 | Butler | A | Vogeler, Allie | Neddeau, Angela | Miller, Madeline | Smith, Aiden | 3:56.36 |
| 6 | Detroit Mercy | A | May, Kye | Campeau, Kennedy | Jackson, Kyri | Tomman, Taylor | 4:01.00 |