| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Jerseyville (Jersey) | A | Mace | Schaffer | Jones | Trexler | 59.48 |
| 4 | Springfield (Lanphier) | A | Lewis | Glover | Manning | Patterson | 56.70 |
| 5 | Piasa (Southwestern) | A | Bachman | Durham | Day | Wilson | 59.14 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Roxana | A | Carlisle | Lohman | Daughtery | Little | 55.54 |
| 2 | Breese (Central) | A | Ahle | Taylor | Detmer | Bassler | 54.34 |
| 3 | Troy (Triad) | A | Yates | Cissell | Schmidt | Frierdic | 52.54 |
| 4 | East St. Louis (Sr.) | A | Graham | Lampley | Jones | Bell | 49.94 |
| 5 | Springfield (Southeast) | A | Chandler-Henry | Rogers | Gant | Jackson | 51.80 |
| 6 | Highland | A | Hadowsky | Wilke | Trover | Thompson | 53.27 |
| 7 | Wood River (East Alton-W.R.) | A | Brown | Sechrest | Nehthery | Douglas | 54.94 |
| 8 | Rochester | A | Walton | Ryan | Stuckey | Tom | 56.41 |