| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Piasa (Southwestern) | A | Durham | Day | Lowis | Wilson | 5:02.14 |
| 4 | Roxana | A | Wirth | Palen | Doyle | Lohman | DNS |
| 5 | Jerseyville (Jersey) | A | Johnson | Womack | Hall | Wendell | 4:50.44 |
| 6 | Staunton | A | Baima | DelValle | Jarden | Roller | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Springfield (Southeast) | A | Chandler-Henry | Smith | Greene | Hood | 4:34.24 |
| 2 | Breese (Central) | A | Detmer | Jansen | Ess | Aparicio | 4:29.23 |
| 3 | Springfield (Sacred Heart-Gr | A | Redpath | Fleischli | Lee | Scarlette | 4:20.16 |
| 4 | East St. Louis (Sr.) | A | Bell | Graham | Lampley | Brown | 4:12.73 |
| 5 | Troy (Triad) | A | Hartoin | Kowalski | Frierdic | Edwards | 4:13.41 |
| 6 | Rochester | A | Lauwerens | Wilber | Hicks | Zeibert | 4:28.37 |
| 7 | Highland | A | Trover | Brindley | Wilke | Loeh | 4:33.40 |
| 8 | Bethalto (Civic Memorial) | A | Dugger | Ferguson | Hannaford | Meiser | 4:36.24 |