| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | Gavin GENISIO | FR | Benton | 4:18.00 |
| 2 | Chase OWENS | JR | Benton | 4:52.00 |
| 3 | Merrett SIMPKINS | JR | Herrin | 4:53.00 |
| 4 | Luke WINKLEMAN | SO | Harrisburg | 4:56.00 |
| 5 | Lance MATTINGLY | SR | Herrin | 5:04.70 |
| 6 | Tucker HORSTMANN | FR | Murphysboro | 5:24.20 |
| 7 | Emilio BAUTISTA | SR | Massac County | 5:40.34 |
| 8 | Jak KOWALCZIK | SO | Massac County | 5:43.22 |
| 9 | Ryan WILSON | JR | Harrisburg | 6:00.00 |
| 10 | Hayeden HUGHES | FR | Frankfort | 6:00.00 |
| 11 | Will HAWTHORNE | SO | Murphysboro | 6:02.89 |