| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Massac County | A | Myers | Bremer | Jacobs | Cohoon | 49.60 |
| 3 | Harrisburg | A | Godsey | Godsey | McIntosh | Fann | 45.50 |
| 4 | Herrin | A | Miles | Williams | Sissom | Chrostoksi | 44.79 |
| 5 | Murphysboro | A | Clemons | Clemons | Clark | Haupt | 44.79 |
| 6 | Benton | A | Frost | Newcomb | Ward | Seidel | 45.60 |