| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | O'Fallon | A | Liebig | Sayles | Holmes | Lehman | 45.67 |
| 4 | Belleville West | A | Brown | Nicholson | Dowell | Ward | 43.56 |
| 5 | Edwardsville | A | Matarelli | Bateman | Hayes | Battle | 42.34 |
| 6 | East St. Louis | A | Akins | Buchanon | McKinnley | Simmons | 43.10 |
| 7 | Collinsville | A | Hills | Hollerbach | Richardson | Rogers | 45.19 |