| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | New Athens | A | Badgett | Castillo | Dachsteiner | Mazariego | 53.00 |
| 3 | Carlyle | A | Missey | Pollmann | Fischer | Gragg | 51.24 |
| 4 | Steeleville | A | Millsap | Buch | Rees | Wasson | 47.64 |
| 5 | East Alton-Wood River | A | Wilcutt | Mason | Skinner | Lane | 48.30 |
| 6 | Red Bud | A | Kinney | Hill | Krebs | Baldwin | 51.34 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Salem | A | Smith | Greene | Ettress | Thornton | 46.50 |
| 2 | Wesclin | A | Monical | Garsnett | Frisse | Evans | 46.02 |
| 3 | Sparta | A | Williams | Richardson | Meridth | Lofton | 45.66 |
| 4 | Columbia | A | Luebbers | Mathews | Jarvis | Judy | 44.17 |
| 5 | Freeburg | A | Murphy | Thompson | Luchtefeld | Merritt | 45.35 |
| 6 | Roxana | A | Osmoe | McBride | Graves | Wells | 45.87 |
| 7 | Breese Central | A | Shubert | Strubhart | Rogers | Tebbe | 46.04 |
| 8 | Chester | A | Schuwerk | Jany | Cowell | Alvarez | 46.50 |