| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | East Alton-Wood River | A | Wilcutt | Lane | Skinner | Mason | 1:48.50 |
| 4 | Breese Central | A | Attaway | Ei | Tebbe | Rogers | 1:43.10 |
| 5 | Chester | A | Caron | Alvarez | Steele | Hopkins | 1:47.00 |
| 6 | Carlyle | A | Missey | Pollmann | Smith | Gragg | 1:50.30 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Steeleville | A | Ingles | Buch | Rubach | Wasson | 1:41.80 |
| 2 | Wesclin | A | Monical | Garsnett | Frisse | Christy | 1:39.00 |
| 3 | Roxana | A | Osmoe | Ellis | Graves | Wells | 1:36.95 |
| 4 | Freeburg | A | Murphy | Merz | Banks | Thompson | 1:34.99 |
| 5 | Columbia | A | Luebbers | Judy | Walton | Blum | 1:35.45 |
| 6 | Sparta | A | Williams | Williams | Meridth | Lofton | 1:38.01 |
| 7 | Salem | A | Smith | Greene | King | Irvin | 1:39.50 |
| 8 | Red Bud | A | Luthy | Baldwin | Jackson | Guebert | 1:42.40 |