| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Skertich | Briggs | Robinson | Crowder | 56.00 |
| 2 | Glenwood | A | Hansborough | Simmons | Gerloff | Caldwell | 54.90 |
| 3 | Belleville West | A | Caldwell | Blue | Hall | Blanchard | 54.10 |
| 4 | Belleville East | A | Howell | Finley | Newsom | Phillips | 53.00 |
| 5 | Edwardsville | A | Stover | Jones | Steinhauff | Flannery | 54.00 |
| 6 | Mascoutah | A | Bower | Housely | Shininger | Lowry | 54.19 |
| 7 | O'Fallon | A | McCoy | Brown | Mohamed | Combs | 55.00 |
| 8 | Murphysboro | A | Davis | Neef | Clark | Glasco-Elerby | DNS |