| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | East Alton-Wood River | A | Brown | Denton | Fulkerson | Nguyen | DNS |
| 3 | Belleville West | A | Singleton | Chapman | Williams | Blue | 2:05.21 |
| 4 | Alton | A | Bruce | Duke | Evans | Banks | 1:59.00 |
| 5 | Columbia | A | Aguiar | Miller | Fredenberg | Theiss | DNS |
| 6 | Civic Memorial | A | Davis | Coffman | Crymes | Vaughn | 2:15.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Glenwood | A | Evans | Schrage | Whewell | Daniels | 1:53.84 |
| 2 | Triad | A | Yates | Cissel | Schmidt | Noyes | 1:52.00 |
| 3 | Mascoutah | A | Lowe | Love | Lee | Beck | 1:49.03 |
| 4 | Belleville East | A | Chism | Armour | Bacon | Tiller | 1:43.14 |
| 5 | O'Fallon | A | Daniels | Perry-Anderson | Scruggs | Pelly | 1:46.90 |
| 6 | Collinsville | A | Clemons | Kohler | Gilmore | Spears | 1:50.50 |
| 7 | Edwardsville | A | Stover | Hockett | Carr | Flannery | 1:52.25 |
| 8 | Murphysboro | A | Oliver | Clark | Maynor | Cook | 1:54.70 |