| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Belleville West | A | Caldwell | Beverly | Green | Traxler | 2:05.21 |
| 2 | Collinsville | A | Skertich | Robinson | Crowder | Parish | 2:00.00 |
| 3 | Mascoutah | A | Boyer | Housely | Athy | Lowry | 1:56.02 |
| 4 | Belleville East | A | Howell | Finley | Galeana | Phillips | 1:50.00 |
| 5 | Glenwood | A | Hansborough | Simmons | Gerloff | Caldwell | 1:53.84 |
| 6 | O'Fallon | A | Mohamed | Brown | McCoy | Glasper | 1:58.00 |
| 7 | Edwardsville | A | Steinhauff | Morningstar | Painter | Taylor | 2:05.00 |
| 8 | Murphysboro | A | Davis | Lopez | Neef | Hunsperger | 2:09.70 |