| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Belleville East | A | Phillips | Smith | Johnson | Newsom | 4:40.00 |
| 2 | Collinsville | A | Dawson | Briggs | Robinson | Jimenez | 4:36.00 |
| 3 | Glenwood | A | Iwanicki | Thompson | Knepler | Hamrick | 4:35.00 |
| 4 | Belleville West | A | Beverly | Faulk | Traxler | Stubbs | 4:21.00 |
| 5 | Edwardsville | A | Morningstar | Stover | Taylor | Lueking | 4:30.00 |
| 6 | Mascoutah | A | Lowry | Kimmle | Benson | Angell | 4:35.84 |
| 7 | O'Fallon | A | Hall | Glasper | Anthony | Miller | 4:40.00 |
| 8 | Murphysboro | A | Davis | Clark | Glasco-Elerby | Nance | 4:56.69 |