| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | East Alton-Wood River | A | Wilcutt | Stone | Mason | Skinner | 1:48.50 |
| 2 | Lanphier | A | Amerson | Williams | Walker | Hall-Burton | 1:40.39 |
| 3 | Belleville East | A | Mcgill | Patton | Young | Smith | 1:39.50 |
| 4 | Cahokia | A | Staples | Devine | DELOACH | Howard | 1:39.00 |
| 5 | Granite City | A | Webb | Rodgers-Parott | Rodgers | Rodgers-Parott | 1:39.40 |
| 6 | Highland | A | Lewis | Gallagher | Schwarz | Beadle | 1:40.10 |
| 7 | Collinsville | A | Gordon | Johnson | Jordan | Williams | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Waterloo | A | Pete | Brewer | Baldwin | Huebner | 1:38.10 |
| 2 | Quincy | A | Rose | O'Hara | Bruening | Bryan | 1:37.00 |
| 3 | O'Fallon | A | Johnson | Suggs | Brady | Doss | 1:35.98 |
| 4 | Springfield | A | Daniels | Hartman | McNeil | Thomas | 1:33.00 |
| 5 | Columbia | A | Luebbers | Blum | Judy | Jarvis | 1:35.45 |
| 6 | Freeburg | A | Murphy | Merz | Banks | Thompson | 1:36.03 |
| 7 | Alton | A | Singleton | Ballinger III | Harris | Pinnix | 1:37.47 |
| 8 | Southeast (Springfield) | A | Daouda | Cummings | Edwards | Turner | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Rantoul | A | Lawson | Anderson | Cobb | Swift | 1:32.24 |
| 2 | Triad | A | Darby | Yohannes | Warren | Cigliana | 1:31.50 |
| 3 | Glenwood | A | Saunders | Sgro | Hendricks | Anderson | 1:30.93 |
| 4 | Carbondale | A | Gleason | Hillard | Dedecker | Threadgill | 1:30.39 |
| 5 | Edwardsville | A | Brnfre | Schmidt | Lakatos | Bush | 1:30.41 |
| 6 | Mt. Vernon | A | Garrett | Hicks | Virden | Davis | 1:31.38 |
| 7 | Belleville West | A | Dowell | Smith-Johnson | Lockwood | Collins | 1:31.56 |
| 8 | Mascoutah | A | Owens | Wells II | Phillips | Thiel | 1:32.39 |