| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Harrisburg | A | Brimm | Hankins | Keener | Morrow | 6:01.00 |
| 4 | Sesser-Valier | A | Ketteman | Castillo | Mitchell | McKee | 5:05.00 |
| 5 | Johnston City | A | Lazorchack | Hewlett | Moulton | Mitchell | 5:20.65 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Carterville | A | Ragan | Brown | Levesque | Edmonds | 4:55.00 |
| 2 | Massac County | A | Turner | Martin | Brugger | Markus | 4:46.26 |
| 3 | Benton | A | Eubanks | Tieffel | Kinkade | Johnson | 4:38.00 |
| 4 | DuQuoin | A | Alongi | Oestricher | Phillips | Heape | 4:18.00 |
| 5 | Herrin | A | Yates | Pickles | Blakey | Mann | 4:27.09 |
| 6 | Marion | A | Engram | Nguyen | Maragni | Edman | 4:40.00 |
| 7 | Fairfield | A | Sagar | Joslin | Hooker | Robbins | 4:51.00 |
| 8 | Carmi-White County | A | Duckworth | Garner | Lamont | Large | 5:04.00 |