| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Freeburg | A | Range | Nissen | Levy | Vahlkamp | 2:07.00 |
| 4 | Columbia | A | Aguiar | Fredenberg | Miller | Theiss | 2:04.00 |
| 5 | Breese (Mater Dei) | A | Toennies | Fuehne | Becker | Strubhart | 2:04.50 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Staunton | A | Trettenero | Baima | Hester | Pfeiffer | 2:04.00 |
| 2 | Madison | A | Gardner | Brown | Belford | Gregory | 2:02.06 |
| 3 | Belleville (East) | B | Galeana | Anderson | Beckwith | Howell | 1:56.00 |
| 4 | Belleville (East) | A | Deere | Newsom | Finley | Polley | 1:50.00 |
| 5 | Murphysboro | A | Green | Maynor | Finch | Oliver | 1:54.70 |
| 6 | Glen Carbon (Father McGivney) | A | Beltramea | Harris | Rakers | Gilbertson | 2:02.00 |
| 7 | Belleville (West) | A | Hall | Johnson | Strong | Caldwell | 2:03.28 |