| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Wood River (East Alton-W.R.) | A | Denton | Brown | Fulkerson | Nguyen | 5:35.00 |
| 4 | Madison | A | Compton | Gardner | Belford | Coulibaly | 5:08.80 |
| 5 | Staunton | A | Pfeiffer | Wright | Hainaut | Schnefke | 5:20.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Freeburg | A | Colgan | Vahlkamp | Spanley | Schneider | 4:55.00 |
| 3 | Murphysboro | A | Green | Gilmore | Gilmore | Shetter | 4:36.69 |
| 4 | Glen Carbon (Father McGivney) | A | Rakers | Terhaar | Gilbertson | Hatley | 4:28.00 |
| 5 | Belleville (East) | A | Deere | Johnson | Polley | Anaya | 4:35.00 |
| 6 | Belleville (West) | A | Johnson | Blue | Hall | Godfrey | 4:45.00 |
| 7 | Belleville (East) | B | Finley | Smith | Gray | Beckwith | 4:55.00 |