| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Greenville | A | Cox | Williams | Rodgers | Siebert | 4:28.44 |
| 2 | Springfield (Sacred Heart-Gr | A | Sanchez | Hanauer | Davis | O'Reilly | 4:01.44 |
| 3 | Wood River (East Alton-W.R.) | A | Wilcutt | Mason | Loeffelman | Green | 3:51.04 |
| 4 | Jacksonville (H.S.) | A | Range | Neice | Finn | Wright | DNS |
| 5 | Salem | A | Ettress | Crane | Thornton | Greene | DNS |
| 6 | Springfield (Southeast) | A | Davis | Jerome | Duffy | Baker | DNS |
| 7 | Roxana | A | Schmidt | Weineke | Smith | Slayden | 4:02.17 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Highland | A | Gallagher | Steinbeck | Mancinas | Brock | 3:41.90 |
| 2 | Bethalto (Civic Memorial) | A | McMurrray | Dutton | Naugle | Henderson | DNS |
| 3 | Troy (Triad) | A | Cigliana | Dudley | Warren | Yohannes | 3:33.80 |
| 4 | East St. Louis (Sr.) | A | Buchanon | Montgomery | Cross | Taylor | 3:24.19 |
| 5 | Breese (Central) | A | Hunt | Shubert | Revermann | Price | 3:29.48 |
| 6 | Rochester | A | Herman | Wilber | Gustafson | Loy | 3:34.12 |
| 7 | Jerseyville (Jersey) | A | Loges | Borkowski | Jones | Hubbell | DNS |
| 8 | Springfield (Lanphier) | A | Robinson | Williams | Patterson | Smith | 3:43.17 |