| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Bethalto Trimpe | A | Flanigan | Zampieri | Davenport | Gares | 59.00 |
| 2 | Illini Middle School | A | Deist | Maxiner | Burch | Judkins | 52.30 |
| 3 | Madison Jr. High School | A | Gardner | Hinds | Willaims | Wilson | 51.90 |
| 4 | Lincoln Middle School | A | Word | Jennings | Whisenand | Smith | 47.90 |
| 5 | Alton Middle School | A | Blake | Whitfield | Prothro | Rounds | 49.50 |
| 6 | Coolidge Junior High | A | Henderson | Dean | Taylor | Brokaw | 52.00 |
| 7 | Edwardsville Liberty MS | A | Thomas | Balentine | Johnson | Williams | 55.00 |