| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Roxana MS | A | Raymer | Ufert | Daughtery | Hill | 59.25 |
| 2 | Liberty Middle School | A | Hartman | Martin | Sadaka | Bartoskevich | 54.80 |
| 3 | Illini Middle School | A | Blasa | Walker | Buttry | Duggan | 54.00 |
| 4 | Lincoln Middle School | A | Cruzin | Thompson | Flannery | Oertle | 52.50 |
| 5 | Gray Alton Middle School | A | Edwards | Myles | Lacey | Jones | 53.30 |
| 6 | Madison Jr. High | A | Travis | Griggs | White | Kelly | 54.50 |
| 7 | Coolidge Junior High | A | Schwaller | Supinski | Allen | Moss | 56.54 |
| 8 | East Alton | A | Minner | Sanders | Moran | Truax | 1:00.30 |