| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Alton (Marquette) | A | Kreitner | Thompson | Redd | Acklin | DNS |
| 2 | Roxana | A | Sims | Smith | Weineke | Ponce | 4:10.00 |
| 3 | Edwardsville (Metro-East Lut | A | Pryor | Schaper | Staake | Schlessinger | 4:00.00 |
| 4 | Madison | A | Kennedy | Wilson Jr. | Trammell | Hayden | 3:40.14 |
| 5 | Bethalto (Civic Memorial) | A | Naugle | Eldridge | Collman | Henderson | 3:43.00 |
| 6 | Wood River (East Alton-W.R.) | A | Wilcutt | Mason | Gould | Jones | 4:05.00 |
| 7 | Glen Carbon (Father McGivney | A | Shearer | Ahring | Moore | Joellenbeck | 4:18.00 |