| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Wood River (East Alton-W.R.) | A | Downs | Peoples | McLagan | Shoemaker | 1:01.00 |
| 2 | Bethalto (Civic Memorial) | A | Coffman | Crymes | Brousseau | Vaughn | 57.78 |
| 3 | Edwardsville (Metro-East Lut | A | Koch | Hughes | Rainey | Sherfy | 56.00 |
| 4 | Glen Carbon (Father McGivney | A | Beltramea | Harris | Gilbertson | Rakers | DNS |
| 5 | Madison | A | Gregory | Belford | Gardner | Brown | 55.56 |
| 6 | Alton (Marquette) | A | Simons | Certa | Wells | Rae | 57.50 |
| 7 | Roxana | A | Lohman | Daugherty | Lohman | Carlisle | 58.56 |