| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Madison | A | Compton | Gardner | Belford | Coulibaly | 5:04.54 |
| 3 | Alton (Marquette) | A | Eccles | Certa | Rister | Johnson | 4:49.20 |
| 4 | Glen Carbon (Father McGivney | A | Hatley | Terhaar | Rakers | Beltramea | 4:37.00 |
| 5 | Bethalto (Civic Memorial) | A | Hannaford | Donaldson | Mann | Meiser | 4:38.00 |
| 6 | Wood River (East Alton-W.R.) | A | Denton | Downs | Shoemaker | Peoples | DNS |
| 7 | Roxana | A | Doyle | Woodruff | Carlisle | Springman | 5:10.00 |