| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Granite City | A | Miller-Brown | Thomas | Posten | Foster | 53.95 |
| 3 | Edwardsville | A | Krebs-Carr | Sanders | Renth | Barton | 51.50 |
| 4 | Alton | A | Bruce | Lewis | Goree | Raglin | 50.50 |
| 5 | Collinsville | A | Clemons | Gary | Kohler | Spears | 50.85 |
| 6 | Triad | A | Yates | Cissel | Allen | Noyes | 52.20 |
| 7 | Highland | A | Trover | Maas | Choat | Thomas | 55.23 |