| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Collinsville | A | Dawson | Clemons | Harris | Jimenez | 4:36.00 |
| 3 | Highland | A | Trover | Brindley | Dortch | Rittenhouse | 4:28.16 |
| 4 | Edwardsville | A | Knoyle | Hockett | Hockett | Coll | 4:20.00 |
| 5 | Triad | A | Niehaus | Gough | Edwards | Noyes | 4:25.00 |
| 6 | Alton | A | Clemons | Paschel | Lumpkins | Bruce | DNS |
| 7 | Granite City | A | Fenoglio | Tanksley | Gerhardt | Davis | 5:01.24 |