| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Granite City | A | Webb | Ivy | Rodgers | Rodgers-Parott | 46.50 |
| 3 | Alton | A | Ballinger III | McClain | Telford | Hardin | DQ |
| 4 | Edwardsville | A | Elliott-Barnes | Brnfre | Schmidt | Jackson | 43.33 |
| 5 | Triad | A | Lightner | Crockarell | Darby | Randoll | 44.00 |
| 6 | Collinsville | A | Edwards | Nelson | Munoz | Richardson | 45.41 |
| 7 | Highland | A | Graham | Beadle | Porter | Wuebbles | 47.40 |