| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Highland | A | Lewis | Gallagher | Mancinas | Irving | 3:48.00 |
| 3 | Alton | A | Kotzamanis | Krafka | Hardin | McClain | 3:37.00 |
| 4 | Edwardsville | A | Ferdinand | Miller | Bateman | Hoeferlin | 3:30.26 |
| 5 | Collinsville | A | Yarbough | Nelson | Munoz | Thorps-Watt | 3:36.86 |
| 6 | Triad | A | Cigliana | Dudley | Gentemann | Pace | 3:37.41 |
| 7 | Granite City | A | Ivy | Davis | Clutts | Davis | 3:53.00 |