| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Highland | A | Wallace | Fridley | Frey | Hollenkamp | 51.98 |
| 3 | Waterloo | A | Rose | Smith | Geller | Morrow | 49.20 |
| 4 | Triad | A | Hudson | McCall | Lightner | Dempsey | 44.94 |
| 5 | Mascoutah | A | Carr | Phillips | Holcomb | Chaffin | 45.82 |
| 6 | Civic Memorial | A | Garrett | LeBlanc | Walters | Paslay | 51.20 |