| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Civic Memorial | A | Hannaford | Vaughn | Mann | Meiser | 4:35.55 |
| 3 | Triad | A | Niehaus | Gough | Edwards | Noyes | 4:19.00 |
| 4 | Mascoutah | A | Williams | Martin | Maschhoff | Drain | 4:13.32 |
| 5 | Waterloo | A | Smith | Johnston | Grohmann | Thorsten | 4:15.73 |
| 6 | Highland | A | Trover | Brindley | Pace | Dortch | 4:28.09 |
| 7 | Jersey | A | Hall | Wilkinson | Finkes | Johnson | 5:06.00 |