| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Freeburg | A | Dircks | Stein | Geluck | Harmon | 4:28.86 |
| 3 | Belleville East | A | Deere | Peppers | Hesker | Armour | 4:24.13 |
| 4 | East St. Louis | A | Glenn | Graham | Lampley | Box | 4:20.00 |
| 5 | Belleville West | A | King | Dunn | Williams | Lewis | 4:10.00 |
| 6 | Mascoutah | A | Williams | Martin | Maschhoff | Drain | 4:16.59 |
| 7 | O'Fallon | A | Daniels | Davis | Pelly | 4:23.00 | |
| 8 | Cahokia | A | Tucker | Millsap | Millsap | Brooks | 4:28.50 |