| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Edwardsville | A | Sanders | Krebs-Carr | Renth | Barton | 51.50 |
| 2 | O'Fallon | A | Lewis-Ramsey | Daniels | Scruggs | Pelly | 50.25 |
| 3 | East St. Louis | A | Glenn | Graham | Lampley | Brown | 50.10 |
| 4 | Belleville West | A | Byrd | Melvin | Dunn | Rivers | 48.10 |
| 5 | Belleville East | A | Chism | Armour | Bacon | Tiller | 49.47 |
| 6 | Collinsville | A | Clemons | Gilmore | Kohler | Spears | 50.19 |
| 7 | Alton | A | Bruce | Carter | Goree | Raglin | 50.30 |