| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Granite City | A | Miller-Brown | Thomas | Herndon | Posten | 2:03.49 |
| 4 | Madison | A | Gregory | Brown | Belford | Gardner | 1:59.00 |
| 5 | Mascoutah | A | Boyer | Lowry | Waddell | Housely | 2:02.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Edwardsville | A | Fry | Krebs-Carr | Barton | Renth | 1:56.67 |
| 2 | East St. Louis | A | Graham | Box | Brown | Lampley | 1:54.00 |
| 3 | Belleville East | A | Unaeze | Finley | Howell | 1:53.00 | |
| 4 | Belleville West | A | Singleton | Chapman | Beverly | Godfrey | 1:49.14 |
| 5 | O'Fallon | A | Lutz | Perry-Anderson | Scruggs | Pelly | 1:52.00 |
| 6 | Rock Island | A | Feigley | Dietz | Campbell | Bumgarner | 1:54.00 |
| 7 | Collinsville | A | Skertich | Gilmore | Briggs | Spears | 1:56.00 |
| 8 | Alton | A | Duke | Lumpkins | Evans | Clemons | DQ |