| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Collinsville | A | Nelson | Epps | Johnson | 46.14 | |
| 2 | O'Fallon | A | Joiner | Bivines | Carter | Hopkins | 44.50 |
| 3 | Belleville East | A | Oluwatimilehin | Foster | Bennett | Mcgill | DQ |
| 4 | Belleville West | A | Dowell | Abram | Young | Nicholson | 43.30 |
| 5 | Edwardsville | A | Bush | Brnfre | Schmidt | Larson | 43.50 |
| 6 | Alton | A | Ballinger III | Telford | McClain | Hardin | DQ |
| 7 | Granite City | A | Poston | Ivy | Rodgers-Parott | Rodgers-Parott | 44.76 |
| 8 | Mascoutah | A | Sutliff | Phillips | Chaffin | Feldt | 52.00 |