| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Granite City | A | Davis | Davis | Clutts | Johnson | 3:58.00 |
| 2 | Collinsville | A | Doria | Munoz | Gordon | Jones | 3:46.40 |
| 3 | Madison | A | Kennedy | Enlow | Moore | Bradley | 3:43.00 |
| 4 | Triad | A | Yohannes | Twyman | Dempsey | Harmon | 3:42.00 |
| 5 | Edwardsville | A | Wilkerson | Kitchen | Helton | Benjarkar | 3:42.34 |
| 6 | O'Fallon | A | Richardson | English | Sheppard | Schram | 3:43.00 |
| 7 | Cahokia | A | Taylor | Moore | Devine | Spiller | 3:52.00 |
| 8 | Belleville East | A | Fuller | Taylor | Wittenuaer | Wright | 4:01.00 |