| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | Brooklyn MCKEE | SR | Sesser-Valier | 2:32.00 |
| 2 | Ellary BLAKEY | FR | Herrin | 2:34.46 |
| 3 | Peyton TIEFFEL | SR | Benton | 2:36.00 |
| 4 | Jacey EUBANKS | SR | Benton | 2:40.00 |
| 5 | Molly ROBERTSON | FR | Carterville | 2:45.00 |
| 6 | Brenna GUSEWELLE | SR | Herrin | 2:48.10 |
| 7 | Mollie MITCHELL | FR | Sesser-Valier | 2:50.00 |
| 8 | Ali HANKINS | SO | Harrisburg | 3:00.00 |
| 9 | Frankie NICHOLES | SO | Harrisburg | 3:01.00 |
| 10 | Megan FLEEGE | SO | Carterville | 3:09.00 |
| 11 | Sedona PACKER | FR | Sesser-Valier | 3:13.00 |
| 12 | Emma FORBY | JR | Goreville | 3:25.00 |
| 13 | Revlyn WILLIS | SR | Frankfort | 3:45.00 |
| 14 | Leslie MORALES | SR | Cobden | 3:50.00 |
| 15 | Jasmine GARCIA | JR | Cobden | 4:00.00 |