| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Harrisburg | A | Trammel | Street | Nicholes | Hankins | 6:01.00 |
| 2 | Carterville | A | Levesque | Edmonds | Hunsaker | Qureshi | DQ |
| 3 | Herrin | A | Yates | Pickles | Mann | Blakey | DNS |
| 4 | Benton | A | Eubanks | Tieffel | Kinkade | Johnson | 4:40.00 |
| 5 | Sesser-Valier | A | Lemons | Packer | Castillo | Kirk | 5:05.00 |