| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Civic Memorial | A | Silva | Wooten | Atteberry | Fonrodona | 46.80 | |
| 2 | Murphysboro | A | Clemons | Kellum | Kellum | Huff-Baker | 46.07 | |
| 3 | Columbia | A | Kronk | Nelson | Donavan | Scott | 45.23 | |
| 4 | Freeburg | A | Merz | Jarvis | Zhu | Thompson | 44.93 | |
| 5 | Granite City | A | Brokaw | Rodgers | Webb | Rodgers-Parott | 44.96 | |
| 6 | Quincy | A | Moore | Altman | Bruening | Brockmiller | 45.76 | |
| 7 | Collinsville | A | Gordon | Munoz | Patterson | Harper | 46.28 | |
| 8 | East Alton-Wood River | A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lanphier | A | Harris | Robinson | Holmes | Brown-Sanchez | 44.26 | |
| 2 | Roxana | A | Coles | Wieneke | Osmoe | McBride | 43.92 | |
| 3 | Glenwood | A | Rayl | Marcordes | Sgro | Saunders | 43.44 | |
| 4 | O'Fallon | A | Johnson | Dowell | Green | Mims | 43.22 | |
| 5 | Belleville West | A | Onyemelukwe | Martin | Smith-Johnson | Sutton | 43.30 | |
| 6 | Carbondale | A | Dedecker | Gleason | Robbins | Burnside | 43.50 | |
| 7 | Springfield | A | Browning | Anderson | Brown | Hartman | 44.06 | |
| 8 | Rochester | A | Barton | Pletsch | Huet | Mrozowski | 44.30 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Belleville East | A | Hardy | Dewalt | Martin | Mitchell | 42.90 | |
| 2 | St. Mary's (St. Louis) | A | Strong | Washington | Hendricks | Roberts | DNS | |
| 3 | Mascoutah | A | Mack | Hippard | Stoltz | Feldt | 42.19 | |
| 4 | Edwardsville | A | Brnfre | Lakatos | Wilson | Lane | 42.07 | |
| 5 | East St. Louis | A | Buchanon | Wren | Montgomery | Taylor | 42.08 | St. Clair County Meet |
| 6 | Triad | A | Darby | Crockarell | Warren | Yohannes | 42.34 | |
| 7 | Alton | A | Singleton | Elliott-Barnes | Harris | McClaine | DQ | |
| 8 | Cahokia | A | Baxtron | Lyons | Midgett | Pennington | 43.22 |